📚 Excited to share my latest publication as an author in the Springer Nature volume: RNA Nanotechnology and Therapeutics: Methods and Protocols
📖 Chapter 18: Production and Testing of RNA Origami Anticoagulants
Co-authored with Carson Key, Kristen Froehlich, and Thom LaBean

This chapter outlines detailed protocols for designing, producing, and evaluating RNA origami-based anticoagulants that use thrombin RNA aptamers. These programmable RNA nanostructures show promising therapeutic potential with high specificity, reversible activity, and improved pharmacokinetics — foundational to the RNA origami platform we're building at Helixomer.

🔬 We explore how self-folding, single-stranded RNA origami can deliver superior anticoagulant effects compared to free aptamers or DNA nanostructures, including using 2′F-modified nucleotides, heat-annealing protocols, and aPTT assays for activity testing.

📌 Check out the chapter here:
🔗 Springer Book Link

#RNAorigami #Anticoagulant #AptamerTherapeutics #Biotech #NucleicAcidNanotechnology #Helixomer #PrecisionMedicine #RNA